ZFIN Image: Chetty et al., 2017, Fig. 1

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Figure Caption

Fig. 1

Inhibition of Vegfr signaling by SU5416 chemical treatment decreases arterial and venous marker expression. (A-P) In situ hybridization analysis showing a decrease in arterial and venous vascular endothelial markers upon SU5416 treatment starting at the 50% epiboly stage (5.3 hpf), as compared to DMSO treated control embryos. Arterial markers cldn5b (A and B), notch3 (C and D), efnb2a (E and F), and deltaC (G and H) as well as venous markers flt4 (I and J), dab2 (K and L), ephb4 (M and N), stab1l (O and P), are downregulated in SU5416 treated embryos. (Q) Quantification of selected markers by qRT-PCR. Markers with high expression outside of the vasculature were excluded from qRT-PCR analysis. * indicates p<0.05 and ** indicates p<0.01, ±SEM is shown. Arrows indicate the dorsal aorta and arrowheads indicate the posterior cardinal vein. Lateral view of 24hpf embryos, anterior to the left. Numbers of embryos showing displayed phenotype are listed.

Acknowledgments

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Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.