IMAGE

Fig. 2

ID
ZDB-IMAGE-170425-4
Source
Figures for Delfino-Machín et al., 2017
Image
Figure Caption

Fig. 2

Enteric precursors are absent in baz1 mutants and supernumerary neurons in trunk and tail do not express markers of non-sensory neuronal types.

A,B) phox2b expression at 72 hpf in wild-types (A) is restricted to the enteric neuron progenitors, but is absent from baz1 embryos (B). Tyrosine hydroxylase (TH) immunofluorescence reveals differentiated sympathetic neurons at 7 dpf in WT (C-E, I-K), whereas these are much reduced in baz1 mutants (F-H, L-M). White arrows indicate a subset of TH+ neurons. Inset in J represents enlargement of boxed area to show autonomic neuron chain more clearly. Occasional baz1 mutants show anti-TH immunofluorescence in neurons (*) associated with the PLLn (O-Q). DIC (C,F,I,L,O) and immunofluorescent TH images (D,G,J,M,P) are merged in panels E,H,K,N,Q respectively. sb, swim bladder; vs, ventral stripe. R) Quantitation (mean ± s.d.) of anti-TH positive sympathetic neurons in WT (N = 16) and baz1 mutants (N = 16). * indicates significant difference (two-tailed t test, p<0.001) Scale bar, 50 μm(C).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One