ZFIN ID: ZDB-IMAGE-170208-32
Figures for Hu et al., 2016

Figure Caption/Comments:

Fig. 6

N70-glycosylation is necessary for the secretion of Leg1b but not Leg1a.

(A) Western blot analysis of secretion of Leg1a, Leg1aN70A, Leg1b and Leg1bN70A. Mutating N70 to A70 does not affect the secretion of Leg1aN70A but does affect Leg1bN70A. Total protein was extracted from cell pellets or supernatant. HA-tagged RNaseI was used as a control. Western blot was repeated three times.(B) Co-immunostaining of Leg1a or Leg1aN70A with the cis- and medial-Golgi marker Giantin in HepG2 cells transfected with WT leg1a and leg1aN70A plasmids, respectively. (C) Co-immunostaining of Leg1b or Leg1bN70Awith Giantin showed that Leg1b is secreted (upper panels) but Leg1bN70A is retained in the cis-Golgi network (lower panels). (D) Co-immunostaining of Leg1b or Leg1bN70A with the ER marker PDI in HepG2 cells transfected with WT leg1b and leg1bN70A plasmids, respectively. The enlarged view of the area highlighted with dashed lines are shown on the right.

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