ZFIN Image: Miranda-Rodríguez et al., 2017, Fig. S3

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Figure Caption

Fig. S3

Myosin Light Chain phosphorylation is affected when RhoA/ROCK pathway is inhibited. Embryos were injected either with RhoA inhibitor (C3) or ROCK inhibitor (h1152) and as control embryos were injected with buffer. At 4-cell stage inmunodetection for myosin light chain phosphorylation (MyoP) was performed. (A and B) Inhibition of RhoA with C3 (n=24) showed the distribution of MyoP at the center of the embryo and diminished compared to buffer (n=11) injected embryos of 2 independent experiments. (C and D) Inmunodetection of MyoP of embryos injected with h1152 (n=41) showed less positive signal compared to buffer (n=32) injected embryos of four independent experiments.

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Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.