IMAGE

Fig. S3

ID
ZDB-IMAGE-160927-39
Genes
Source
Figures for Liu et al., 2016
Image
Figure Caption

Fig. S3

fscn1a morphants exhibit defects in epiboly progression and endoderm formation.

(a) Representative bright-field images of 4 ng fscn1a mis-MO1 and MO1 injected embryos at 8.5 hpf and bud stages. Lateral views with dorsal to the right. (b-c) Knockdown of fscn1a by injection of 25 ng fscn1a MO2 dramatically reduced the expression of the early endodermal markers sox32 (b) and sox17 (c) at the 75% epiboly stage. Panels are shown in dorsal view with anterior to the top. (d-e) The expression of the mesendodermal markers is normal in fscn1a morphants. Embryos injected with mis-MO1 (4 ng) or fscn1a MO1 (4 ng) at the one-cell stage and harvested at the 75%-epiboly stage for in situ hybridization with gsc (d) and ntl (e) probes. Dorsal views with anterior to the top. Scale bar, 200 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.