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Fig. 2
Generation of a mosaic fin by a blastema transplant. (A) The procedure of the blastema transplantation and mosaic analysis. Donor blastema cells were taken from the double-transgenic zebrafish of Tg(hsp70l:dnfgfr1) and Tg(Olactb:loxP-dsR2-loxP-EGFP); the latter transgene drives ubiquitous expression of DsRed2 (Yoshinari et al., 2012). The regenerated fins are a chimera of host cells and transplanted donor cells. (B) A whole-mount fluorescent view of the mosaic fin at 5days post-transplantation (dpt). The fin contains progenies of the transplanted cells expressing the dsRed2. Scale bar: 500µm. (C) A longitudinal tissue section of the mosaic fin at 5dpt stained with an antibody against Zns5, a marker of osteogenic cells (green), and DAPI (blue). The transplanted cells (red) mostly gave rise to mesenchymal cells (11 Zns5-positive cells out of 226 DsRed2-positive cells, counted from 12 sections, from independent transplantations in four different fish). Scale bar: 50µm.