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Fig. 5
Apical Par proteins regulate primary ciliogenesis. (A-C) Lateral images of living 3dpf Tg(µact::Arl13b-GFP) embryos, focused on the trunk spinal cord. Scale bar: 20µm. Brackets mark the spinal cord (sc) and outlined boxes show digital enlargements of primary cilia. The long motile cilia in ventral spinal cord were not used for these analyses. Scale bar: 1µm. (D) Graph showing cilia length. Data represent the mean±s.e.m. (n=30 embryos from 3 independent experiments for each group and 10-30 cilia measured in each embryo). Significance calculated using an unpaired t-test. (E) Graph showing cilia length in 3dpf control, pard3 TP MO-injected and prkci TP MO-injected embryos. Data represent the mean±s.e.m. (n=30 embryos from 3 independent experiments for each group, and 10-30 cilia measured in each embryo). Significance calculated using an unpaired t-test. (F-M) Confocal images captured from time-lapse movies of dividing cells in the trunk spinal cord of a control embryo, beginning at 24hpf. Time elapsed from the start of the movie is indicated on each panel. Cilia are labeled by Arl13b-EGFP and cell membranes are marked by membrane-tethered mCherry. Scale bar: 10µm. Outlined boxes show digital enlargements of cilia. Scale bar: 1µm. (N) Graph showing average cilia length (µm) over 120min and outlined boxes show digital enlargements of cilia. The 0min time point corresponds to the 88min time point in J. Slope shows the rate of cilia growth is greater in miR-219 MO-injected embryos than in controls. Data represent the mean±s.e.m. (n=7 cells from 7 embryos for both conditions). Significance calculated using unpaired t-test. **P=0.01959, *P=0.0527.