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Fig. S3

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ZDB-IMAGE-160715-9
Source
Figures for Richardson et al., 2016
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Figure Caption

Fig. S3

The epidermis around closing full-thickness wounds lacks apoptotic keratinocytes. (A-C) TUNEL staining on sections through unwounded (A.B) and wounded (C) trunk region, 4 hpw, with (B) and without (A,C) DNase treatment to induce DNA fragmentation, as formerly described (Fischer et al., 2014). No TUNEL-positive cells are detectable in the epidermis 1 mm from the wound, indicating that the thinning of the epidermis in this region is not due to keratinocyte apoptosis. Similar results were obtained via immunofluorescent labelling of activated caspase 3 (data not shown). Abbreviation: mc, melanocyte.

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