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Fig. 5
Transgene expression in the forebrain of Tg(lhx5:GFP)b1205 embryos and larvae. (A,A′) Dorsal views of the habenula of a 4dpf Tg(lhx5:GFP)b1205 fish labeled with anti-GFP only (A) or anti-GFP (green) and anti-SV2 (magenta) antibodies (A′). GFP+ axon terminals (green) innervate all habenula neuropil domains visualized with SV2 (magenta). (B-C′) Lateral views of the forebrain of a Tg(lhx5:GFP)b1205 fish at 48 hpf (B,B′) and 4dpf (C,C′). (B) GFP+ cells and processes in 48 hpf Tg(lhx5:GFP)b1205. Note that the tract of the habenular commissure is labeled at this stage (tHC, white arrow). Yellow arrow points to putative prethalamic eminence/vENT. (B′) Image shown in (B) has been converted to gray scale and false colored areas show the different presumptive domains. GFP+ cells and processes form a continuous band at the telencephalic-diencephalic boundary (B), extending from presumptive prethalamus (PTh, labeled in green), through the putative prethalamic eminence/vENT (vENT, labeled in yellow) and preoptic regions (PO, labeled in blue). A number of GFP+ neurons are also present in the olfactory bulb (OB, labeled in pink). (C) GFP+ cells and processes in 4dpf Tg(lhx5:GFP)b1205 fish. Note that at this stage the main afferent tracts to the habenula, the stria medullaris (StMed) and the tract of the habenular commissure (tHC) are visible. Yellow arrow points to putative prethalamic eminence/ vENT. (C′) Image shown in (C) has been converted to gray scale and false colored areas show the different presumptive domains. There are a number of GFP+ cells rostrally in the OB (pink area), a few GFP+ cells and fibers in Dp (purple). Prethalamus, vENT and preoptic regions are again labeled in green, yellow and blue respectively. Scale bars: All 100 µm.