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Fig. 1
DiI labeling of connections of the habenula in adult zebrafish. (A) Brain schematic showing the levels of sections in (C-H). Images are dorsal view of whole brain with anterior to the left (B) or transverse sections (C-H) through the brain of adult zebrafish after DiI application to the left or right habenula (orange area in A and pink in B). (B) Location of a typical DiI application into the right habenula (arrow). Dotted lines mark the limits of both habenulae. (C) Labeled mitral cells located in the dorsomedial region of the mitral cell layer in the olfactory bulb (arrowheads). Level of this section corresponds to Figure 2A. Inset: Detail of two mitral cells, showing characteristic dendritic arbors (arrows). (D) Labeled cells located bilaterally in the ventrolateral telencephalon (vENT, arrowheads) and neuropil formed by mitral cell collaterals in Dp (arrow). Note that although the vENT is labeled bilaterally, the ipsilateral nucleus (right) is more intensely labeled. Level of this section corresponds to Figure 2C. (E,F) Confocal images of the cells located in the vENT. At rostral levels (E), small neuronal cell bodies (arrowheads) are densely distributed among a dense network of cell processes (arrow; this level of the nucleus corresponds to Figure 2C). At caudal levels (F), more sparsely distributed neurons (arrowheads) surround the lateral forebrain bundle (this level of the nucleus corresponds to Figure 2D). (G) Labeled cells in a nucleus located in the prethalamus that may be the rostrolateral nucleus (this section corresponds to level represented in Figure 2E). (H) Labeled neurons (arrowheads) and beaded axons (arrows) located in the posterior hypothalamic lobe (this section corresponds to level shown Figure 2G). Scale bars: 500 µm in (B), 50 µm in (C,E,F,H) and 100 µm in (D,G).