ZFIN Image: Koenig et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Coalescence of angioblasts into the supra-intestinal artery and interconnecting vessel formation. Confocal microscope timelapse images of live Tg(kdrl:mCherry);Tg(fli1a:EGFP) embryos beginning at approximately 56 hpf. Note that subintestinal vessel progenitors have low mCherry expression and appear mostly green. (A and B) indicate region of interest displayed in other panels. (C-J) Maximum intensity projections of confocal images at selected time points. (C′-J′) Selected confocal sections were combined in a maximum intensity projection of the same region as in C-J to more clearly visualize SIA. At 56 hpf, portions of the SIA are already visible with few connections to the SIV (C,C′). Over a period of 24 h (D-J′), cells from the PCV contribute to the further development of the SIA while branches from the SIV, indicated by arrowheads, extend toward the SIA and form interconnecting vessels (ICVs). Anterior-left, dorsal-top.

Acknowledgments

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Reprinted from Developmental Biology, 411(1), Koenig, A.L., Baltrunaite, K., Bower, N.I., Rossi, A., Stainier, D.Y., Hogan, B.M., Sumanas, S., Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein, 115-27, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.