IMAGE

Fig. 1

ID
ZDB-IMAGE-160404-1
Source
Figures for Koenig et al., 2016
Image
Figure Caption

Fig. 1

Cells from the posterior cardinal vein form the sub-intestinal vein, and the supra-intestinal artery. Confocal microscope timelapse images of live Tg(kdrl:mCherry);Tg(fli1a:EGFP) embryos beginning at approximately 25 hpf. (A and B) indicate region of interest displayed in other panels. Note that subintestinal vessel progenitors have very low kdrl:mCherry expression and appear green while endothelial cells within the axial vessels show both mCherry and GFP expression. (C) A thickened cell within the ventral wall of the PCV is observed which delaminates in (D) (arrows). Note the large heterogeneity of transgene expression within the wall of the PCV with mCherry negative cells interspersed next to mCherry positive cells. (E) Multiple SIV progenitors are observed (arrows) which still make contacts with the PCV. (F) SIV progenitors (arrows) acquire elongated shape with some of them still contacting the PCV. (G) SIV progenitors start to coalesce into a primordial vessel. Some still make contacts with the PCV (arrow). (H) SIV progenitors have largely coalesced into a vessel. Notice a new cell emerging from the PCV (arrow). (I) Multiple cells emerge from the PCV (arrows) and start coalescing into the SIA. Filopodia bridging the SIA and SIV are apparent. (J) Separate SIA and SIV primordial vessels are apparent. Anterior-left, dorsal-top.

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Reprinted from Developmental Biology, 411(1), Koenig, A.L., Baltrunaite, K., Bower, N.I., Rossi, A., Stainier, D.Y., Hogan, B.M., Sumanas, S., Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein, 115-27, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.