ZFIN ID: ZDB-IMAGE-160205-14
Figures for Etard et al., 2015

Figure Caption/Comments:

Fig. S5

aTg(-505/-310 (unc45b)gata2:gfp) embryos were injected with hsp90a morpholinos (hsp90a-mo) or with hsp90a-mo together with a mix of CRISPR RNA directed against hsf1 and cas9 mRNA (hsp90a-mo + guide hsf1). We note a decrease of GFP in the hsf1/hsp90a double knock-down compared with the hsp90a single morphant. b-b′ Injection of the plasmid encoding a Hsf1-mOrange fusion protein does not activate the unc45b promoter. Expression of TFP reporter in hsf1-morange injected embryos (+hsf1:morange) (a, b) is as high as in uninjected tg(1.8unc45b:tfp) embryos (cont) (a). c-h′ Heat shock triggers unc45b up-regulation. -505/-310(unc45b)gata2:gfp injected embryos (c, d, c′, d′), Tg(-505/-310 (unc45b)gata2:gfp) (e, f, e′, f′) and tg(-1.8unc45b:tfp) (g, h, g′, h′) transgenic embryos were raised at 28 °C until 48 hpf and heat shock at 37 °C for 12 hours (d, d′, f, f′, h, h′). Embryos shown in (c, c′, e, e, g, g′) were kept at 28 °C. The heat shock embryos show up-regulation of GFP in skeletal and cardiac muscles, demonstrating that the regulatory sequences of unc45b react to heat shock.

Figure Data:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.