IMAGE

Fig. 1

ID
ZDB-IMAGE-150423-36
Genes
Antibodies
Source
Figures for Windner et al., 2015
Image
Figure Caption

Fig. 1

Tbx6 induces mesp-b paralogs and ripply1 before the onset of myogenesis in lateral paraxial mesoderm. (A-E) Labeling for tbx6, mesp-ba, ripply1 and myoD mRNAs, and for Tbx6 and myosin heavy chain (MF20) proteins at the 10-somite stage. Unbroken lines indicate most recently formed somites (SI-SIII); dashed lines indicate future somites (S0, S-I, S-II) in the anterior pre-somitic mesoderm (PSM). Scale bar: 100µm. (F) Anti-Myc immunoprecipitation from Tg(hsp70l:tbx6myc) embryos 1h after heat shock and subsequent sequencing reveals Tbx6Myc genomic binding proximal to mesp-ba, mesp-bb and ripply1. Shown are 35-kb windows encompassing significant peaks in reads per million (RPM) (supplementary material Table S1). Black asterisks indicate correspondence with T-box binding sites identified by Cutty et al. (2012). Red asterisks indicate peaks confirmed by ChIP-PCR (supplementary material Fig. S2). (G) Top sequence motif found in Tbx6Myc binding peaks (supplementary material Table S2). (H) Schematic showing spatial and temporal correlation of tbx6, mesp-ba and ripply1 expression with the onset of dermomyotome and myotome (slow and fast fiber) development (adapted from Stellabotte and Devoto, 2007).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development