ZFIN Image: Sasaki et al., 2014, Fig. S7

Image

Figure Caption

Fig. S7

Effects of spns1 and p53 knockdowns on embryonic SA-β-gal activity in p53 and spns1 mutants, respectively. (A) Effect of spns1 knockdown on embryonic senescence in p53 mutants. The impact of transient spns1 knockdown on SA-β-gal induction was determined in spns1 MO-injected tp53^zdf1/zdf1 animals at 72 hpf. Standard control MO was used for control injections. Scale bar, 250 μm. (B) Effect of p53 knockdown on embryonic senescence in spns1 mutants. The impact of transient p53 knockdown on SA-β-gal induction was determined in p53 MO-injected spns1^hi891/hi891 animals at 72 hpf, followed by the MO injections. Inverse p53 MO (inv. p53 MO) was used for control injections. Scale bar, 10 µm. (C) Quantification of the SA-β-gal intensities shown in (A). Quantification of data presented in panel A (n = 12) is shown in the right graph; the number (n) of animals is for each genotype with MO. (D) Quantification of the SA-β-gal intensities shown in (B). Quantification of data presented in panel B (n = 12) is shown in the right graph; the number (n) of animals is for each morphant in genotype. Error bars represent the mean ± S.D., *p<0.005; ns, not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.