ZFIN ID: ZDB-IMAGE-140626-28
PCR analysis of microsporidia-infected D. rerio. Two fish with fluorescent granulomas
were commercially assayed by PCR for microsporidial DNA from Pseudoloma neurophilia and Pleistophora hyphessobryconis
(IDEXX RADIL, Columbia, MO). (A) Lck:EGFP transgenic fish with fluorescent nodules, wasting, and abnormal
spine curvature imaged using FITC/GFP filter (emission spectrum 500–550 nm). (B) Identical fish imaged using TRITC
filter (emission 575–625 nm). Like other animals harboring fluorescent nodules (Fig. 1), lesions coincide using either filter,
chiefly in skeletal muscle of flanks. (C) PCR results visualized by capillary electrophoresis: P. neurophilia was detected in
CNS and skeletal muscle of both fish (top, lanes 1–4). No template (lane 5, negative control) shows no product; positive
control (lane 6) yielded band of identical size. P. hyphessobryconis, a Microsporidian known to infect muscle, was not
detected in skeletal muscle of either fish (bottom, lanes 2 and 4). CNS, central nervous system; FITC, fluorescein isothiocyanate;
PCR, polymerase chain reaction.
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.