ZFIN ID: ZDB-IMAGE-140626-27
Figures for West et al., 2014

Figure Caption/Comments:

Fig. S2 Histopathologic analysis of microsporidia-induced granulomas in D. rerio. Zebrafish with fluorescent nodules were fixed, sectioned, and stained using several methods: (A) Low-power H&E stain of lesion in skeletal muscle. Skin and subcutaneous tissue are seen at the top of the panel. Skeletal muscle runs longitudinally above the lesion and is shown in the cross section surrounding the lesion. Yellowboxed region is shown at higher magnification in panel B. (C) High-power image of a lesion stained by PAS. The lesion itself is PAS + , as are individual spores within the granuloma as denoted by black lines. (D) High-power image of a PTAH-stained lesion showing proteinaceous debris and fibrin deposits within the granuloma. Yellow boxes highlight myonecrosis, with striations of degenerating skeletal muscle still visible. Intact skeletal muscle at the bottom of the image shows striations clearly. (E) The 10X-magnified gram stain of lesions within skeletal muscle. Lesion in upper right corner is shown at 20 · magnification in (F). (G) GMS stain highlighting carbohydrates in the fungal cell wall. Section shown in panel G is from an adjacent section to that shown in panel E. All 3 lesions are GMS+ at 10 · magnification. (H) The 20 · magnification of the GMS+ lesion shown in the upper right corner of panel G.

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