ZFIN ID: ZDB-IMAGE-140530-16
Proximity ligation assay indicates close physical proximity at the subcellular level. (A) Immunolabeling of
acetylated tubulin. Acetylated tubulin fills up the cytoplasm and labels the kinocilium. (B) DIC channel. (C) Merge of panels
A and B. (D) Proximity ligation assay of acetylated tubulin and Harmonin results in a restricted signal localized in the basal
region of the hair cell, as indicated by the yellow arrowheads. (E) DIC channel. (F) Merge of panels D and E. (G) Proximity
ligation assay of acetylated tubulin and Harmonin in ush1c mutant results in the absence of signal. (H) DIC channel. (I)
merge of panels G and H. (J) Proximity ligation reaction of gamma tubulin and Harmonin resulted in no signal. (K) DIC
channel. (L) Merge of panels J and K. (M) Immunolabeling of gamma tubulin showing a nonspecific signal filling the cytoplasm.
(N) DIC channel. O: merge of panels M and N. Scale bar: 5µm.
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.