ZFIN ID: ZDB-IMAGE-140530-15
Proximity ligation control: Assay of nonspecific binding between proximity ligation probes in the absence
of one of the primary antibodies during the reaction in WT conditions. (A,D,G,J,K,M,P) The proximity ligation
reaction was performed with only one primary antibody: Goat anti-Cdh23 (A), rabbit anti-Harmonin (D), goat anti-Harmonin
(G), rabbit anti-Ift88 (J), mouse anti-Myo7a (K), goat anti-Sec13 (M), or rabbit anti-Sec23 (P). (B,E,H,N,Q) single
DIC channel showing a lateral view of the anterior macula. (C,F,I,L,O,R) Merged panels. Nonspecific signal was detected
when only one primary antibody was added to the reaction. Scale bar: 5µm.
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