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Fig. S1

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ZDB-IMAGE-130827-30
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Figures for Wythe et al., 2013
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Fig. S1

Wnt/β-Catenin signaling is not active in early arteries and does not regulate early Dll4 expression. (A) Conservation between murine and opossum Dll4 with location of fragment 1 (F1) indicated. Transgenic analysis of F1-lacZ (E9.5) is below. (B) Representative whole-mount F1-lacZ transgenic embryo. (C) Wholemount (left) and transverse sections (right) of BAT-gal embryos at E8.5 (n=5) and E9.5 (n=4). Dorsal aorta (arrow); cardinal vein (caret). (D) Whole-mount (left) and zoomed-in (right) Axin2-d2EGFP embryos at E8.5 (n=6) and E9.5 (n=8). (E) Whole-mount (left) and transverse sections (right) of Axin2nlacZ/+ embryos at E8.5 (n=3) and E9.5 (n=5). (F) Vascular patterning in endothelial-specific Ctnnb1 (β-catenin) loss-of-function (ECKO) embryos was normal, as visualized by Cre-mediated recombination of a GFP reporter allele (Rosa26-mTomato-lox-stop-lox-mGFP [mTmG]) (left). The dorsal aorta (arrow) and cardinal vein (caret) are indicated. A zoomed-in view (right) of the brain vasculature demonstrates a less remodeled vascular plexus (indicated by arrows) in the ECKO (n=7) embryonic brain compared to control (n=10) embryos. (G) Dll4 in situ hybridization indicates normal expression in the dorsal aorta (arrow) of Ctnnb1 ECKO embryos (n=4). (H) No defects in arteriovenous morphology in Ctnnb1 null (n=5) embryos were observed, as assessed by ink injection (as ink filled the entire dorsal aorta (arrows)) and H&E histology of serial cross-sections through the rostral and caudal planes of the dorsal aortae and cardinal veins. Dorsal aorta (arrow); cardinal vein (caret). Scale bars: 500 μm (B-H, whole-mount), 100 μm (C-H, sections and zoom ins).

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Reprinted from Developmental Cell, 26(1), Wythe, J.D., Dang, L.T., Devine, W.P., Boudreau, E., Artap, S.T., He, D., Schachterle, W., Stainier, D.Y., Oettgen, P., Black, B.L., Bruneau, B.G., and Fish, J.E., ETS Factors Regulate Vegf-Dependent Arterial Specification, 45-58, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell