Gpr125-Cherry alone did not affect PCP components localization in late blastulae (4-5 hpf). (A-A′′) Animal pole views of a blastula co-injected with 110 pg fzd7-CFP and 300 pg gpr125-Cherry RNA. (B-B′′) Animal pole views of a blastula co-injected with 60 pg gpc4/kny-GFP and 300 pg gpr125-Cherry RNA. (C-C′′) Animal pole views of a 300 pg gpr125-Cherry and 150 pg mGFP RNA-injected blastula immunostained for GFP, RFP and Vangl2.
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