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Fig. S4

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ZDB-IMAGE-130801-12
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Figures for Teslaa et al., 2013
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Fig. S4 Zic2a translation-blocking morpholino rescues craniofacial defects associated with elevated Zic2a levels. Alcian blue-stained cartilage in heat-shocked Tg(hsp70l:Gal4VP16);Tg(11XUAS:zic2aYFP) embryos at 5 dpf. (A–D) HS-induced expression of Zic2aYFP at 10 hpf results in severely shortened and hypoplastic anterior cartilages in uninjected embryos (67/67 embryos, 1 exp.; shown in C,D) (E–H) Zic2aYFP misexpressing control morphants display severe shortening and hypoplasia comparable to uninjected siblings (23/24, 2 exp.; shown in G,H). (I,J) Zic2aYPF misexpressing embryos injected with Zic2a morpholino were either wild-type (28/135, 2 exp.) or displayed variable craniofacial defects including shortening of anterior cartilages with an incorrectly angled hyoid arch (58/136, 2 exp.; shown in I,J) or more severe hypoplasia (12/136, 2 exp.; not shown). Zic2a overexpressor morphants did not display detectable amounts of the induced Zic2aYFP fusion protein (132/136, 2 exp.). (K) Penetrance of cartilage defects at 5 dpf following zic2aAUGMO injection and Zic2aYFP induction at 10 hpf. A,C,E,G,I are ventral views anterior to the left. B,D,F,H,J are lateral views.

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Reprinted from Developmental Biology, 380(1), Teslaa, J.J., Keller, A.N., Nyholm, M.K., and Grinblat, Y., Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development, 73-86, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.