ZFIN ID: ZDB-IMAGE-130719-11
Development of CagA+ transgenic zebrafish. (A) Ubiquitous CagA-EGFP fusion protein expression driven by the b-actin promoter. (B) Ubiquitous CagAEPISA-EGFP fusion protein expression driven by the b-actin promoter. (C) Intestinal CagA-EGFP fusion protein expression driven by the i-fabp promoter. (D) RT-PCR of dissected larval intestine showing expression of cagA and the housekeeping control gene RPL13 at 6 dpf. (E) Quantitative RT-PCR of dissected adult intestines showing relative expression levels of cagA transcript in transgenic lines at 1 year of age. Expression levels were normalized to SDHA and β-actin; bars indicate mean
± s.d. of biological triplicates. Scale bars: 500 μm.
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