Morpholinos targeting mnx family genes are specific and effective in knocking down protein. Lateral views of two spinal hemisegments, segment boundaries denoted by diagonal lines, of uninjected and control MO-injected Tg(mnx1:GFP) embryos labeled for antibodies against Mnx1 (A, D), Mnx2a (B, E), and Mnx2b (C, F). Embryos injected with mnx1 MO lack Mnx1 antibody labeling (G), but maintain Mnx2a (H) and Mnx2b (I) antibody labeling. Embryos injected with mnx2a MO lack Mnx2a antibody labeling (K), but maintain Mnx1 (J) and Mnx2b (L) antibody labeling. Embryos injected with mnx2b MO lack Mnx2b antibody labeling (O), but maintain Mnx1 (M) and Mnx2a (N) antibody labeling. Scale bar: 20 μm.
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