ZFIN Image: Behra et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Immuno-staining in neuromasts of 5 dpf Tg (tnks1bp1:EGFP) larvae. A. B. Confocal images of two different neuromasts (1 and 2) double-stained with anti-GFP (green, first and last columns) and anti-Myosin VI (red, second and last columns). The GFP positive cells (green) are the accessory cells (i.e. a combination of supporting cells and mantle cells). Around the apical pole of hair cells, accessory cells form a precisely organized honeycomb like annular structure (white arrows in A and B, first columns). Hair cells send their tightly packed hair bundles through the openings in the top, (white arrowheads in A, middle panel). C. Schematic cross-section (left image) and dorsal view (right image) of a neuromast illustrating the respective position of the accessory cells (cytoplasm light green and nucleus dark green) and the hair cells (cytoplasm dark red and nucleus light red). D. Cryosection of a neuromast, immunolabeled with an anti-GFP antibody. GFP expression is excluded from all hair cells (nucleus and cytoplasm) and found in all accessory cells, comprising the supporting and mantle cells (white stars). - 10 microns in A and B, 5 microns in D.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.