ZFIN Image: Kizil et al., 2011, Fig. 6

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Figure Caption

Fig. 6 Morpholino-mediated gene knock-down using CVMI is dose-dependent.

(A) The dose-response analyses were performed on dorsal regions of telencephalic sections. PCNA immunohistochemistry coupled to DAPI nuclear counterstaining on 500 μM control morpholino-injected (B), 500 μM PCNA morpholino-injected (C), 250 μM PCNA morpholino-injected (D), 100 μM PCNA morpholino-injected (E), 50 μM PCNA morpholino-injected (F) brains. (G) Graph depicts the relative amount of PCNA-positive cells in dorsal telencephalon after every dose in control and PCNA morpholino-injected brains. Scale bars 50 μm. N = 3 adult fish for every dose.

Acknowledgments

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