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Fig. 1 The development of the zebrafish caudal vein plexus. (A-I) Confocal images of caudal vasculature at different developmental stages. (A-E) Untreated control Tg(kdrl:GFP) embryos. (F-I) Tg(kdrl:GFP) embryos treated with 10 μM aplexone beginning at 10 hpf. The red arrow in A indicates a primary ISV. The yellow arrowhead in B indicates venous angiogenic sprouts. Yellow arrows in G,H indicate filopodia that fail to mature into angiogenic sprouts. Insets in B,G,H are magnified images of the areas indicated by the yellow arrowhead and arrows. Red bars in B and E indicate the distance between the axial artery and vein. (J,K) Optical cross-sections of blood vessels in control (J) and aplexone-treated (K) embryos at 48 hpf. Yellow bars in D and I indicate the position where the cross-sections were extracted. (J′,K′) Schematic drawings of the cross-sections in control (J′) and aplexone-treated (K′) embryos. Red represents the dorsal aorta and blue the caudal vein. (L,M) Confocal images of blood vessels in the trunk of control (L) and aplexone-treated (M) Tg(kdrl:GFP) embryos. Images were taken at 48 hpf. (L′,M′) Schematic drawings of L and M. Red and blue represent the artery and vein, respectively. Horizontal vessels are the dorsal aorta and posterior cardinal vein. Vertical vessels are intersegmental vessels.