Whole mount in situ hybridization of Polycomb PRC1 components during caudal fin regeneration at 48 hpa (a–f). Longitudinal sections of caudal fins demonstrating PRC1 components expression during regeneration at 48 hpa (g–k). Ubiquitous presence of Ub-H2AK119 in caudal fin detected by immunohistochemistry (l). IHC was performed on PFA fixed longitudinal sections of fins at 48 hpa. The epidermis (e) and mesenchyme (m) are indicated in each panel. Antibody signal (brown) and hematoxylin counterstain (blue) are shown.
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