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Fig. 2

ID
ZDB-IMAGE-091214-56
Source
Figures for Rieger et al., 2009
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Figure Caption

Fig. 2 Cadherin-2 is a likely candidate to mediate GC migration in the zebrafish cerebellum.

(A, B) Transverse view of the developing zebrafish cerebellum after ISH shows cadherin-2 expression during stages of prominent GC migration (black arrowheads point out dorsal most cerebellar region where migrating GCs are positioned). (C, D) Lateral view high magnification of the cerebellum. Migrating GCs in gata1:GFP transgenic embryos co-express cadherin-2 at high levels (white arrows). (E, F) Combined TOPRO and Bodipy-Ceramide staining of the cerebellum (lateral view) in WT (E) and pac-/- embryos (F). The rudimentary cerebellum in pac-/- embryos still forms a rhombic lip, as demarcated by expression of atoh1a (G: WT, H: pac-/- embryos, dorsal views, anterior is left). (I–N) Migration routes of GCs in the cerebellum. Dorsal view time-lapse sequence of migrating GCs in gata1:GFP transgenic embryos. (I–K) WT GCs migrate preferentially toward and along the MHB to form two clusters: the dorsomedial corpus cerebelli (orange dashed circles in K) and ventro-lateral eminentia granularis (blue dashed circle in K, see Video S2). (L–N) In pac-/- mutants, GCs of the dorsally forming corpus cerebelli remain close to the URL and settle in ectopic clusters (see Video S3). The MHB and URL are delineated with a dashed and solid line, respectively. Anterior is left. cb, cerebellum; hb, hindbrain; MHB, midbrain-hindbrain boundary; URL, upper rhombic lip.

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