IMAGE

Fig. 5

ID
ZDB-IMAGE-091113-83
Source
Figures for Hart et al., 2009
Image
Figure Caption

Fig. 5 Requirement of TRF3 and TAF3 for expression of mouse Mesp1. A: ChIP analysis monitoring occupancy of TRF3, TBP, RNA polymerase II (POL2) or, as a negative control (C), an irrelevant protein (yeast Gal4) on the Mesp1 and Actin promoters in mouse E10.5 embryos. B: ChIP analysis monitoring promoter occupancy of TRF3, TBP, TAF3, and POL2 in mouse F9 embryonal teratocarcinoma cells. C: RT-PCR analysis of Trf3 and Mesp1 gene expression in F9 cells at 0, 1, 3, or 6 days after addition of retinoic acid (RA). D: RNAi. Trf3 double-stranded RNA (dsRNA) or, as a control, GFP dsRNA, was transfected into F9 cells and 48 hr later Trf3, Mesp1, Tbp, and Gapdh expression were monitored by RT-PCR analysis. E: Taf3 dsRNA or control (GFP) dsRNA was transfected into F9 cells and 48 hr later Taf3, Trf3, Mesp1, and Actin expression were analyzed by RT-PCR.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.