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Fig. 2 Northern blots of zebrafish embryonic and adult RNA hybridized with Zash probes. Zash-1a (top) and Zash-1b (bottom) anti-sense riboprobes were hybridized to a filter containing RNAs extracted from embryos at times (hr) indicated above each lane. The rightmost lane in each case contained poly(A)+ RNA from adult fish. A parallel gel containing the same samples was stained with ethidium bromide. The amounts of ribosomal RNA in each sample were equivalent, indicating that differences in hybridization were not due to variations of RNA loaded on the gel.
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Reprinted from Developmental Biology, 166, Allende, M.L. and Weinberg, E.S., The expression pattern of two zebrafish achaete-scute homolog (ash) genes is altered in the embryonic brain of the cyclops mutant, 509-530, Copyright (1994) with permission from Elsevier. Full text @ Dev. Biol.