Fig. 3

Fig. 3 Df(LG23)wnt1^w5 is a deficiency for wnt1 and wnt10b. (A) PCR analysis of wild type (+/+) and homozygous mutant DNA (Df/Df). Loci indicated are from the LN54 radiation hybrid panel (see http://zfish.uoregon.edu/ZFIN for mapping panel information). (B, C) Lateral views of 5-dpf embryos, anterior to the left. Melanocytes form normally in Df^w5 embryos (arrows), somites appear normally shaped (asterisks), and the gut tube appears normal (arrowheads), indicating that tissues from all three germ layers are able to form normally. Defects appear most noticeably in the head, as Df^w5 embryos have reduced eyes (e). (D–G) Wild type and Df^w5 embryos at the prim-25 stage (36 hpf). Viewed laterally (D, E), wild type and homozygous mutants are almost indistinguishable (arrows indicate the cerebellum). When viewed dorsally (F, G), an increased distance between the medial edges of the MHB fold is visible in the mutants (double arrows). Additionally, cell death is apparent in the optic tectum (arrow in G). (Insets) Acridine orange staining of 24-hpf embryos. (H, I) Sagittal sections of 48-hpf embryos. Arrow indicates abnormal structure in the Df^w5 tegmentum– hindbrain interface. tc, tectum; tg, tegmentum; hb, hindbrain; tel, telencephalon; di, diencephalon; ov, otic vesicle; v, ventricle.