Branching defects in developing caudal veins in reg6 embryos. (A) A wild-type TG(fli1:EGFP)y1 embryo at 30 h postfertilization (hpf) (33°C) shows numerous branches (yellow arrows) in the developing caudal vein. (B) These branches develop into a plexus within a few hours and the plexus persists for several days during embryogenesis (a 40-hpf embryo is shown). (C) Bright field image of (B). (D) The developing caudal vein in reg6;TG(fli1:EGFP)y1 embryo at 30 hpf (33°C) shows fewer branches (yellow arrow) and a developing sinus (red arrow). (E) By 40 hpf, the developing caudal veins in reg6;TG(fli1:EGFP)y1 embryos become swollen and form a sinus (red arrow). (F) Bright field image of (E); red arrow, developing sinus. Anterior to the left and dorsal up. hpf, hour post fertilization. Scale bars, 100 μm.
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