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Fig. 7

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ZDB-IMAGE-080925-8
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Figures for Del Bene et al., 2008
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Fig. 7 Disruption of the Nuclear Anchor to Dynactin Phenocopies the moks309 Mutation

(A and B) Expression of the Müller glia marker GS is reduced in the retina of larvae injected with a syne2a-MO (B) compared to control MO-injected larvae (A). Scale bars, 100 μm.

(C and D) Representative examples of sections of 5 dpf retinas overexpressing a control vector (C) or a dominant-negative Syne2a (KASH, [D]) under the control of a heat-shock promoter. The cells that express the constructs are identified by the expression of GFP marker (green). Clones of cells that express the syne2a dominant-negative construct preferentially generate GCL neurons. Scale bars, 50 μm.

(E) Quantification of the KASH overexpression results, showing the distribution of clones in the three retinal nuclear layers. *p < 0.01, **p < 0.005. Error bar indicates SEM.

(F) A model of the mechanism that couples INM with graded Notch activation.

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell