kid and kis allow effective ablation of the germ line without affecting embryonic morphology. (A and D) Control embryos injected with 2 pg of the antidote RNA developed without apparent somatic defects and are morphologically similar to embryos injected with 1 pg of kid-nos1 3′UTR plus 1 pg of kis-glo (G and J). Reduction in the number and lack of GFP-labeled (B, E, H, and K) or vasa RNA-labeled (C, F, I, and L) germ cells at 24 and 48 hpf. Red arrows indicate the germ cells, and blue arrows indicate the regions where PGCs are normally found in the fluorescent images (B, E, H, and K). The same regions are boxed and magnified in C, F, I, and L.(M) A quantitative representation of the number of germ cells after different treatment at 24 and 48 hpf, as determined by in situ hybridization using vasa antisense RNA probe.
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