The formation of the zebrafish gonads does not depend on colonization by PGCs. (A–C) Hematoxylin–eosin-stained paraffin sections of 20- and 35-dpf fish derived from embryos injected with control MO. (A1–C1) Magnifications of the boxed regions of the gonad in A–C. Blue arrows indicate the gonads labeled as testis (T), ovary (O), or undifferentiated (left blank). (D and E) Sections of 20- and 35-dpf fish injected with 200 pg of dnd MO. Arrows indicate gonadal tissue, which is detected at the correct position (D1 and E1).
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