Visualization of calcified axial skeletal structures in developing zebrafish embryos. (A, B) Embryos at 8 and 9 dpf, respectively, showing that calcification of vertebrae number 4 appears earlier than vertebrae numbers 2 and 3. Vertebrae 1, 2, 3, and 4 are indicated. (C) Embryos at 9 dpf, showing the calcification initiation site of the vertebrae, which commences at the boundaries of each segment.
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