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Fig. 5 Endothelial cell behaviour during formation of the DLAV. The figure depicts different ISVs within a single embryo. (A–C) ZO-1 pattern during vessel fusion. Confocal projections of 30 hpf fli1:EGFP fish (red) labeled with anti ZO-1 (green). (A) ZO-1 accumulates at the contact site of two putative tip cells, which then crawl over each other resulting in a small (B) oval-shaped ZO-1 pattern, which eventually expands to an extended oval (C). (A′–C′) Schematic representation of the ZO-1 pattern shown in panels A–C. (D, E) Picture series of supplementary movies 1 and 2 showing dynamic cell behaviour during ISV formation. Movie (D) shows a tip cell expressing mCherry in a flk1:EGFP embryo. Within 5 h, the tip cell migrates dorsally and expands in anterior and posterior direction to fuse and form the DLAV. After first contact (D″) tip cells “crawl” over each other to some extent (arrow in panel D′″). Movie (E) shows a single stalk cell migrating dorsally to contribute to the DLAV (arrow in panel E′″). Scalebars: (A–C) 20 μm; (D–D′″) 50 μm; (E–E′″) 100 μm.
Reprinted from Developmental Biology, 316(2), Blum, Y., Belting, H.G., Ellertsdottir, E., Herwig, L., Lüders, F., and Affolter, M., Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo, 312-322, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.