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Fig. 1 Complex distribution of junctional proteins in ISV. (A) Cellular model of ISVs according to Childs et al. (2002). (B) Putative distribution of cell junctions (red rings) in an ISV in which endothelial cells are arranged in a head-to-tail fashion forming a seamless tube. (C–E) Confocal projection of 36 hpf fli1:EGFP (red) embryos labeled with an anti-ZO-1 antibody (green). ZO-1 protein is mostly detected as two “stripes” (arrows in panels C and D) along the stalk of the ISV, and also in parts of the DLAV. Sometimes, ZO-1 is absent from short regions of the ISVs, including the DLAV (E, arrowheads). (F–I) ZO-1 outlines single cells. 30 hpf wild-type fish injected with flk1:RAS-GFP (red) plasmid and labeled with anti ZO-1 antibodies (green). (F and G) Putative single stalk cells are entirely lined by ZO-1. (H and I) Putative tip cells show ring-like ZO-1 pattern at their base, where they make contact with stalk cells (arrows). Abbreviations: da: dorsal aorta, nc: notochord, sb: somite boundary. Scalebars: 20 μm.
Reprinted from Developmental Biology, 316(2), Blum, Y., Belting, H.G., Ellertsdottir, E., Herwig, L., Lüders, F., and Affolter, M., Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo, 312-322, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.