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Fig. 1 Analysis of the enhancer trap line CLGY713 and identification of duplicate sfrp1 genes. Whole mount fluorescent imaging of live embryos (A–C) or YFP-immunostaining (D) of line CLGY713 was performed at the indicated stages. Organisation of the sfrp1a genomic locus (formerly sfrp1) and CLGY virus integration site (E). Amino acid sequence alignment of Sfrp1 proteins from zebrafish and mouse (F). The single letter code for amino acids is used. Amino acid numbering is indicated on the right. Identical and similar residues are indicated in black and grey, respectively. The conserved N-terminal cystein-rich domain (CRD) and C-terminal netrin (NTR) homology are shown. Phylogenetic tree of the full-length Sfrp1, Sfrp2 and Sfrp5 proteins (G). The degree of relatedness is indicated by the length of the vertical lines. Numbers indicate bootstrap support for the nodes. Ci, Ciona intestinalis; Dr, Danio rerio; hb, hindbrain; Hs, Homo sapiens; hpf, hours post-fertilisation; Mm, Mus musculus; ov, optic vesicle; ret, retina; ss, somite stage; teg, tegmentum; Tn, Tetraodon nigroviridis; Tr, Takifugu rubripes; vfb, ventral forebrain; 2°pllp, secondary posterior lateral line primordium.
Reprinted from Gene expression patterns : GEP, 6(8), Pezeron, G., Anselme, I., Laplante, M., Ellingsen, S., Becker, T.S., Rosa, F.M., Charnay, P., Schneider-Maunoury, S., Mourrain, P., and Ghislain, J., Duplicate sfrp1 genes in zebrafish: sfrp1a is dynamically expressed in the developing central nervous system, gut and lateral line, 835-842, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns