ZFIN Image: Lien et al., 2006, Fig. 4

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Figure Caption

Fig. 4 pdgf-b Expression Is Upregulated in Regenerating Zebrafish Hearts. (A) RT-PCR analysis of pdgf-b and pdgfr-α. β-actin was used as a loading control. Expression of pdgf-b began to increase at 3 dpa and lasted until 14 dpa during zebrafish heart regeneration. pdgfr-α was expressed in both sham-operated and regenerating hearts; its expression level does not change. (B) In situ hybridization using a radioactive antisense probe showing pdgf-b expression in sham-operated and regenerating hearts at 7 dpa. Expression of pdgf-b was localized to the wound site. A radioactive sense probe was used as a negative control. The dashed red line marks the amputation plane.

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Acknowledgments

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