Knockdown of Tsg function enhances dorsalization in mini fin (tolloid) mutants. (A) Wild-type and (B) mfn homozygous mutant embryo at 1 dpf. Arrowheads indicate normal and deficient ventral tail tissue. Injection of 12.5 ng MO5 into mfn/mfn embryos results in a moderate class 3 dorsalization (C). The increase in phenotypic strength is seen at the four-somite stage with the lateral expansion of somites in injected (E) mfn homozygotes (arrowheads) compared with uninjected (D).
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