FIGURE

Fig. 3

ID
ZDB-FIG-201124-22
Publication
Frame et al., 2020 - Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production
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Fig. 3

IL1β Mediates HSPC Production (A) Generation of Tg(hsp70l:il1b) embryos. (B) runx1/cmyb expression (36 hpf) in the dorsal aorta with or without il1b overexpression (actIL1β; 32 hpf) in chimeric injected embryos. (C) Phenotype distribution plot of runx1/cmyb expression scored in embryos from (B) (n ≥ 117/condition). (D) actIL1β induces runx1 in whole Tg(hsp70l:il1b) embryos by qPCR (48 hpf, n = 5, mean ± SEM). (E) HSPCs quantified by flow cytometry (FC) in Tg(flk1:dsRed;cmyb:GFP;hsp70l:il1b) embryos compared with actIL1β− controls (48 hpf). Error bars indicate SD. (F) actIL1β (32 hpf) induces expression of il1b and common targets (n ≥ 3, 48 hpf, mean ± SEM). (G) Relative frequency of neutrophils in 120 hpf Tg(mpx:GFP;hsp70l:il1b) embryos heat-shocked at 32 hpf. Error bars indicate SD. (H) WISH images of cmyb expression in the kidney marrow (arrows) at 120 hpf, after actIL1β induction at 32 hpf. CFP eye signal was used to identify the presence of the hsp70l:il1b transgene. (I) Quantification of kidney marrow area in embryos from (H). Error bars indicate SD. Scale bars, 100 μm. ∗p < 0.05, ∗∗p < 0.01. See also Figure S3.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 55(2), Frame, J.M., Kubaczka, C., Long, T.L., Esain, V., Soto, R.A., Hachimi, M., Jing, R., Shwartz, A., Goessling, W., Daley, G.Q., North, T.E., Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production, 133-149.e6, Copyright (2020) with permission from Elsevier. Full text @ Dev. Cell