FIGURE

Fig. 3

ID

ZDB-FIG-201117-10

Publication

Xia et al., 2020 - Slc39a5-mediated zinc homeostasis plays an essential role in venous angiogenesis in zebrafish

Other Figures

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Fig. 3

slc39a5 morphants have impaired endothelial cell proliferation and migration in the CVP region. (a–b) PH3 (a) and BrdU (b) immunostaining of control and slc39a5 morphant Tg(fli1a:eGFP) embryos, with magnified views of the CVP showing co-localization of GFP fluorescence and PH3 immunoreactivity (arrows). (c) Summary of BrdU-positive cells in the CVP of control and slc39a5 morphants. ***p < 0.001. (d) Representative sequential images of the CVP in control and slc39a5 morphant Tg(fli1a:eGFP) embryos. For each embryo, two separate endothelial cells are indicated with a white asterisk and a yellow asterisk. Note that 0 h corresponds to 32 hpf. (e) Summary of the migration distance of endothelial cells from 32 to 35 hpf in the CVP of control and slc39a5 morphant Tg(fli1a:eGFP) embryos. (f) Representative images of TUNEL staining in the CVP of control and slc39a5 morphant Tg(fli1a:eGFP) embryos. (g–h) Representative images (g) and summary (h) of H2AX immunostaining in the CVP of control and slc39a5 morphant Tg(fli1a:eGFP) embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-slc39a5
Knockdown Reagent:	MO1-slc39a5
Observed In:	caudal vein plexus apoptotic process caudal vein plexus endothelial cell migration caudal vein plexus endothelial cell proliferation
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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