FIGURE

Figure 7.

ID

ZDB-FIG-200530-34

Publication

Lee et al., 2020 - Tgfb3 collaborates with PP2A and Notch signaling pathways to inhibit retina regeneration

Other Figures

All Figure Page

Back to All Figure Page

Figure 7.

Tgfb3 acts upstream of Notch signaling to inhibit MG proliferation.

(A) Top illustration is experimental time line. Bottom panels show mCherry immunofluorescence on retinal sections from either injured tp1:mCherry or hsp70:tgfb3;tp1:mCherry transgenic fish. Asterisk and box region shows the injury site. Note forced Tgfb3 expression stimulate mCherry expression. (B) Quantification of data mCherry+ cells shown in (A). (C) Top is experimental time line. Bottom panels show BrdU immunofluorescence on retinal sections from injured and heat shock-treated Wt and hsp70:tgfb3 fish treated +/- RO4929097. (D) Quantification of data in (C). (E) qPCR analysis of dll4 and hey1 RNA expression using total retinal RNA from Wt and hsp70:tgfb3 fish that were given a 1 hr heat shock (HS) treatment before sacrifice. Values are normalized to Wt control. Error bars are SD. *p<0.05, ***p<0.001. Scale bar is 50 microns.

Expression Data

Genes:	dll4 hey1
Fish:	WT mi51Tg
Condition:	heat shock, surgical manipulation: retina
Anatomical Term:	retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT jh11Tg mi51Tg jh11Tg; mi51Tg
Conditions:	heat shock, surgical manipulation: retina chemical treatment by environment: RO4929097, heat shock, surgical manipulation: retina
Observed In:	Muller cell cell population proliferation retina
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife