ZFIN ID: ZDB-FIG-200518-37
Lu et al., 2020 - Reissner fibre-induced urotensin signalling from cerebrospinal fluid-contacting neurons prevents scoliosis of the vertebrate spine. Biology Open   9(5) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
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Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 3 sspo mutants show loss of urp gene expression from CSF-cNs. (A) A wild-type embryo, showing urp1 expression in CSF-cNs (arrows). (B) An sspo-mutant embryo, showing reduction in levels of urp1 expression from CSF-cNs (arrows). (C) A wild-type embryo, showing urp2 expression in CSF-cNs (arrows). (D) An sspo-mutant embryo, showing almost complete lack of urp2 expression from CSF-cNs (arrows). Embryos shown in A–D are representative of a minimum of 25 embryos analysed for each genotype for each urp gene. (E) A wild-type embryo, showing urp1 expression in CSF-cNs after exposure to epinephrine (arrows). (F) An sspo-mutant embryo, showing urp1 expression in CSF-cNs after exposure to epinephrine (arrows). Note that expression level is indistinguishable from wild type. Embryos from mating of sspo-heterozygous fish were exposed to epinephrine in two independent experiments (25 embryos each). Since all embryos showed similar levels of urp1 expression, 13 embryos were randomly genotyped after in situ hybridisation from the second experiment, of which three were mutants. (G) A wild-type embryo, showing urp2 expression in CSF-cNs after exposure to epinephrine (arrows). (H) An sspo-mutant embryo, showing restoration of urp2 expression in CSF-cNs after exposure to epinephrine (arrows). Embryos from mating of sspo-heterozygous fish were exposed to epinephrine in two independent experiments (25 embryos each). 16 embryos from the second experiment, with urp2 expression in the spinal cord, were genotyped after in situ hybridisation, of which five were mutants. All embryos depicted in A–H were at 26 hpf. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
urp1 AB standard conditions Long-pec cerebrospinal fluid contacting neuron ISH
sspoicm13/icm13 standard conditions Long-pec cerebrospinal fluid contacting neuron ISH
sspoicm13/icm13 chemical treatment by environment: (R)-adrenaline Long-pec cerebrospinal fluid contacting neuron ISH
urp2 AB standard conditions Long-pec cerebrospinal fluid contacting neuron ISH
sspoicm13/icm13 standard conditions Long-pec cerebrospinal fluid contacting neuron ISH
sspoicm13/icm13 chemical treatment by environment: (R)-adrenaline Long-pec cerebrospinal fluid contacting neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sspoicm13/icm13 standard conditions Long-pec cerebrospinal fluid contacting neuron urp1 expression decreased amount, abnormal
Long-pec cerebrospinal fluid contacting neuron urp2 expression decreased amount, abnormal
sspoicm13/icm13 chemical treatment by environment: (R)-adrenaline Long-pec cerebrospinal fluid contacting neuron urp1 expression amount, ameliorated
Long-pec cerebrospinal fluid contacting neuron urp2 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open