ZFIN ID: ZDB-FIG-200406-149
Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis. Cell communication and signaling : CCS   18:45 Full text @ Cell Commun. Signal.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4

ER stresses in copper-stressed and BFA-treated embryos. A Phenotypes of representative copper-stressed embryos with or without BFA. BFA treatment destroys the COPII function and induces ER stresses, A7, measurement of eye diameters of the embryos. A1-A6, lateral view, anterior to the left. B Protein levels of P­eIF-2α in copper-stressed embryos with or without BFA (B1) and the quantification of protein levels of P­eIF-2α in each sample (B2). C Western blot detection of XBP1-s in copper-stressed embryos (C1) and the quantification of protein level in each sample (C2). D Immunostaining of PDI (ER marker) in copper-treated embryos. D1-D3, merged images of immunostaining of PDI (green) and DAPI staining (blue); D4, D5 and D6 were magnified domains of white boxes marked in D1, D2 and D3, respectively. D1- D6, sagittal slides in eyes domain. Scale bar: A1-A6, 0.2 mm; D1-D9, 50 μm; D10-D12, 10 μm. **, P < 0.01; *, P < 0.05

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-xbp1 WB AB control Protruding-mouth whole organism
WB AB chemical treatment: copper(2+) Protruding-mouth whole organism
WB AB chemical treatment: copper atom Protruding-mouth whole organism
Ab3-eif2a WB AB control Protruding-mouth whole organism
WB AB chemical treatment: brefeldin A Protruding-mouth whole organism
WB AB chemical treatment: copper(2+) Protruding-mouth whole organism
WB AB chemical treatment: brefeldin A, chemical treatment: copper(2+) Protruding-mouth whole organism
WB AB chemical treatment: copper atom Protruding-mouth whole organism
WB AB chemical treatment: brefeldin A, chemical treatment: copper atom Protruding-mouth whole organism
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: brefeldin A Protruding-mouth whole organism Ab3-eif2a labeling increased amount, abnormal
Day 4 eye decreased diameter, abnormal
AB chemical treatment: copper(2+) Protruding-mouth whole organism Ab1-xbp1 labeling increased amount, abnormal
Protruding-mouth whole organism Ab3-eif2a labeling increased amount, abnormal
Day 4 eye decreased diameter, abnormal
AB chemical treatment: brefeldin A, chemical treatment: copper(2+) Protruding-mouth whole organism Ab3-eif2a labeling increased amount, abnormal
Day 4 eye decreased diameter, abnormal
AB chemical treatment: copper atom Protruding-mouth whole organism Ab1-xbp1 labeling increased amount, abnormal
Protruding-mouth whole organism Ab3-eif2a labeling increased amount, abnormal
Day 4 eye decreased diameter, abnormal
AB chemical treatment: brefeldin A, chemical treatment: copper atom Protruding-mouth whole organism Ab3-eif2a labeling increased amount, abnormal
Day 4 eye decreased diameter, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell communication and signaling : CCS for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Commun. Signal.