ZFIN ID: ZDB-FIG-200306-122
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Days 14-20 to Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 14-20 to Days 30-44

Fig. 7

Defects in the migration and polarity of PCs in reln mutants. Sagittal sections of the brain from WT (A-C, n= 4) and relnΔ7/Δ7 (D-H, n= 4) 15-dpf larvae, and from WT (I, n= 3) and relnΔ7/Δ7 (J, n= 3) 30-dpf fish, were stained with anti-Pvalb7 (magenta) and anti-Vglut1 (green) antibodies, and Hoechst (cyan). Typical cerebellum images are shown. The ventral limit of the cerebellum is indicated by a dotted line. (Ba, Ca, Da, Ea, Fa, Ga, Ha) High magnification images of the PCs marked by “a” in B–H. The Pvalb7 and Vglut1-double positive region marks the ML. Migrating PCs were detected in the GCL in WT, and they extended a neurite (primary dendrite) toward the pial side (Ba, Ca, n= 4). Many of the migrating cells extended one or multiple neurites in aberrant directions in the relnΔ7/Δ7mutants (Ea, Fa, Ga, Ha, n= 4). At 30 dpf, most of the PCs had reached the PCL in WT (I), whereas many ectopic PCs were detected in the GCL in relnΔ7/Δ7 mutants (J, n= 3). The abbreviations are described in the legend for Fig. 1. Scale bars: 20 μm in A (applies to A-H); 100 μm in I (applies to I-J).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pvalb7 Ab1-pvalb7 AB standard conditions Days 14-20 granular layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 14-20 Purkinje cell layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 30-44 granular layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 30-44 Purkinje cell layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 relnnub23/nub23(AB) standard conditions Days 14-20 granular layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 14-20 Purkinje cell layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 30-44 granular layer corpus cerebelli Purkinje cell IHC
Ab1-pvalb7 Days 30-44 Purkinje cell layer corpus cerebelli Purkinje cell IHC
slc17a7a Ab1-slc17a7 AB standard conditions Days 14-20 cerebellar granule cell axon IHC
Ab1-slc17a7 Days 30-44 cerebellar granule cell axon IHC
Ab1-slc17a7 relnnub23/nub23(AB) standard conditions Days 14-20 cerebellar granule cell axon IHC
Ab1-slc17a7 Days 30-44 cerebellar granule cell axon IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
relnnub23/nub23(AB) standard conditions Days 14-20 cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Days 14-20 granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Days 14-20 Purkinje cell neuron projection disoriented, abnormal
Days 30-44 cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Days 30-44 granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.