ZFIN ID: ZDB-FIG-200306-120
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Ectopic Bergmann glial cells (BGs) in reln mutants. (A–H) Aberrant localization of BGs in reln mutants. Sagittal section of adult (96 dpf) SAGFF(LF)251A; UAS:GFP brains, which express GFP in BGs, harboring WT (control, n= 5) or homozygous reln mutant (relnΔ7/Δ7n= 5) alleles were stained with anti-Pvalb7 (magenta) and anti-GFP (green) antibodies. (B-D, F–H) High magnification images of the boxes in A and E. (I) Ratio of the BG (GFP+) area in the GCL to the total BG area in controls and relnΔ7/Δ7mutants. Fluorescence images were captured of every fourth section (14 total sections in each fish). The GFP+ area in the Pvalb7-negative GCL layer was measured and divided by the total GFP+ area in the cerebellum. *p< 0.05 (Mann-Whitney test). Scale bars: 300 μm in A (applies to A and E); 50 μm in B (applies to B-D and F–H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkuasgfp1aTg ; nkSAGFFLF251AEt(AB) standard conditions Adult Bergmann glial cell IHC
relnnub23/nub23 ; nkSAGFFLF251AEt ; nkuasgfp1aTg(AB) standard conditions Adult Bergmann glial cell IHC
pvalb7 Ab1-pvalb7 nkuasgfp1aTg ; nkSAGFFLF251AEt(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 relnnub23/nub23 ; nkSAGFFLF251AEt ; nkuasgfp1aTg(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
relnnub23/nub23 ; nkSAGFFLF251AEt ; nkuasgfp1aTg(AB) standard conditions Adult Bergmann glial cell inserted into granular layer corpus cerebelli, abnormal
Adult granular layer corpus cerebelli has extra parts of type Bergmann glial cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.