ZFIN ID: ZDB-FIG-200206-35
Silbernagel et al., 2018 - The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function. FASEB journal : official publication of the Federation of American Societies for Experimental Biology   32(11):6159-6173 Full text @ FASEB J.
Anatomical Terms:
Stage: Protruding-mouth

Figure S8

Cardiac expression of VAPB in embryonic zebrafish hearts, rescue experiments after MO-VAPB knock-down and overexpression of VAPBP56SA) Immunostaining with an α-VAPB antibody (see Supplementary Methods) showing an uniform expression of VAPB in embryonic zebrafish hearts at 72 hours post fertilization. A, atrium. V, ventricle.B) Two representative embryonic zebrafish hearts stained against VAPB, either after injection with control morpholinos or with the VAPB knock-down morpholino, respectively. The embryonic zebrafish heart was co-stained against DAPI to show the cell nuclei. The experiments illustrate an efficient knock-down of VAPB and specificity of the antibody. C) After morpholino-antisense mediated VAPB knock-down (MOVAPB), the injection of cRNA encoding for either VAPB or D) VAPA rescued the reduction in embryonic heart rates. Data were obtained from four and three independent batches of injections, respectively. E) The injection of cRNA for the human VAPA or VAPB did not alter embryonic heart rates. Moreover, injection of VAPBP56S cRNA is likely to not act in a dominant-negative manner on zebrafish Ichannels, as the heart rates are not altered. Data were obtained from three independent batches of injections. All data are presented as mean ± s.e.m.. The number of experiments (n) are indicated in the bar graphs. ***, P < 0.001 using an unpaired Welch́s T-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
vapb Ab1-vapb TU standard conditions Protruding-mouth atrium IHC
Ab1-vapb Protruding-mouth cardiac ventricle IHC
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal FASEB journal : official publication of the Federation of American Societies for Experimental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ FASEB J.